A RP-HPLC-DAD-APCI/MSD method for the characterisation of medicinal Ericaceae used by the Eeyou Istchee Cree First Nations.

Mon, 02/11/2013 - 21:59 -- Tracy Wysote
TitleA RP-HPLC-DAD-APCI/MSD method for the characterisation of medicinal Ericaceae used by the Eeyou Istchee Cree First Nations.
Publication TypeResearch
Year of Publication2010
AuthorsSaleem A, Harris CS, Asim M, Cuerrier A, Martineau L, Haddad PS, Arnason JT
KeywordsChromatography, High Pressure Liquid, Ericaceae, Humans, Indians, North American, Limit of Detection, Plants, Medicinal
Abstract

INTRODUCTION: Ericaceae medicinal plants are traditionally used by the Eeyou Istchee Cree and other northern peoples of North America to treat type 2 diabetic symptoms. Because of the importance of phenolics as potential cures for degenerative diseases including type 2 diabetes, an analytical method was developed to detect them in the leaf extracts of 14 Ericaceae plants. OBJECTIVE: To develop an optimised method which is applicable to a relatively large number of Ericaceae plants using their leaf extracts. For this purpose phenolics with a wide range of polarity, including a glucosylated benzoquinone, two phenolic acids, three flavanols, a flavanone, a flavone and five flavonols, were included in this study. METHODOLOGY: Characterisation of phytochemicals in extracts was undertaken by automated matching to the UV spectra to those of an in house library of plant secondary metabolites and the authentication of their identity was achieved by reversed phase-high-performance chromatography-diode array detection-atmospheric pressure chemical ionisation/mass selective detection. RESULTS: Twenty-six phenolics were characterised within 26 min of chromatographic separation in 80% ethanol extracts of 14 Ericaceae plants. The calibration curves were linear within 0.5-880 microg/g dry mass of the plant with regression values better than 0.995. The limits of detection ranged from 0.3 for microg/mL for (+)-catechin to 2.6 microg/mL for chlorogenic acid. This is a first study dealing with relatively large number of Ericaceae extracts and is applicable to other plants of same family.

DOI10.1002/pca.1203