Bioassay-guided isolation of the antidiabetic principle from Sorbus decora (Rosaceae) used traditionally by the Eeyou Istchee Cree First Nations.

Tue, 02/05/2013 - 16:44 -- Tracy Wysote
TitleBioassay-guided isolation of the antidiabetic principle from Sorbus decora (Rosaceae) used traditionally by the Eeyou Istchee Cree First Nations.
Publication TypeResearch
Year of Publication2010
AuthorsGuerrero-Analco JA, Martineau L, Saleem A, Madiraju P, Muhammad A, Durst T, Haddad P, Arnason J T
KeywordsAnimals, Anti Diabetic Plant Project, Hypoglycemic Agents, Mice, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Plant Bark, Plant Stems, Quebec, Sorbus, Stereoisomerism, Triterpenes
Abstract

Bioassay-guided fractionation of a crude extract (80% EtOH in H(2)O) of stem bark of Sorbus decora led the isolation of three new pentacycle triterpenes (compounds 1-3). The structures of 1-3 were established on the basis of spectroscopic methods (IR, HREIMS, 1D and 2D NMR) as 23,28-dihydroxyursan-12-ene-3β-caffeate, 23,28-dihydroxylupan-20(29)-ene-3β-caffeate, and 3β,23,28-trihydroxy-12-ursene, respectively. Compound 2 significantly enhanced glucose uptake in C2C12 cells, but compounds 1 and 3 did not. In addition, triterpenoids 4-8, catechin, and epicatechin were also isolated. This is the first comprehensive report of the phytochemical constituents of S. decora since the initial study by Narashmachari and von Rudloff (1962) and includes evaluation of their antidiabetic activity.

DOI10.1021/np1003005